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Title: Functional Display of Ice Nucleation Protein InaZ in Bacterial Ghosts

Speaker: Werner Lubitz, Bird-C, Vienna (Host: Tobias Weidner)

21.05.2019 | Susan Hjort Skyum

Dato tor 23 maj
Tid 15:15 16:00
Sted 1510-435, Aarhus Universitet, Institut for Kemi, Langelandsgade 140, 8000 Aarhus C

Functional Display of Ice Nucleation Protein InaZ in Bacterial Ghosts

Mascha Rauscher, Johannes Kassmannhuber and Werner Lubitz, BIRD-C GmbH, Vienna, Austria

In a concept study the ability to induce heterogeneous ice formation by Bacterial Ghosts (BGs) from E. coli carrying ice nucleation protein InaZ from P. syringae in their cell envelope was investigated by a droplet-freezing assay of ultra-pure water. BGs are defined as empty non-denatured cellular envelopes derived from Gram-negative bacteria which can be modified by addition of new functions (e.g. InaZ). The standard production process is sustainable, simple and short in time resulting in high yields of BGs which could carry significant amounts of immobilized proteins. As determined by the median freezing temperature and cumulative ice nucleation spectra it could be demonstrated that both the living recombinant E. coli and their corresponding BGs functionally display InaZ on their surface. Under the production conditions chosen both samples belong to type II ice-nucleation particles inducing ice formation at a temperature range around -6°C. One advantage for the application of such BGs over their living recombinant mother bacteria is that they are non-living native cell envelopes retaining the biophysical properties of ice nucleation and do no longer represent genetically modified organisms (GMOs). The application of InaZ-BGs as new cloud seeding material is discussed.

Institut for Kemi, Medarbejdere, Offentligheden / Pressen