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Department of Chemistry Seminar Series: Kurt V. Gothelf, iNANO and Dept. of Chemistry, AU (Host: Tobias Weidner)

Folding Double-Stranded DNA with Triplex-forming Oligonucleotides

Info about event


Thursday 14 December 2023,  at 15:00 - 16:00


1514-213, Aud I, Department of Chemistry, Langelandsgade 140

15:00-15:15       Coffee & cake in the foyer
15:15-16:00       Scientific talk in Auditorium I
16:15-17:00       Q&A session for PhD and master students in auditorium I

Speaker: Kurt V. Gothelf, Department of Chemistry, Aarhus University

The spatial organization of genomic DNA is essential for gene compaction and regulation. Nature has evolved sophisticated machinery to organize double-stranded DNA (dsDNA),but engineering tools for this purpose are sparse.
Here, we fold dsDNA into designed shapes by exploiting the sequence-structure relationship of DNA triplexes.1 Triplexforming oligonucleotides (TFOs) bind purines in dsDNA via normal or reverse Hoogsteen interactions.
In our triplex origami methodology, these non-canonical interactions are programmed to compact dsDNA into well-defined objects. The inherent rigidity of dsDNA promotes structural robustness and non-periodic structures are therefore formed with fewer unique starting materials. Triplex-mediated dsDNA folding is methodologically straightforward and orthogonal to Watson-Crick-based methods. Moreover, it enables unprecedented spatial control over dsDNA templates. The presentation will also include other examples of our recent research in nucleic acid chemistry.